About Us
Immunohistochemistry (IHC) is a laboratory technique used to detect antigens in tissue sections through the binding of specific antibodies. The term comes from “immuno” (antibodies) and “histo” (tissue). By combining antibodies with antigens, researchers can visualize specific biomarkers in tissues using staining methods such as immunoperoxidase or immunofluorescence.
There are two main approaches:
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Direct method – a one-step process in which a labeled antibody directly binds to the target antigen.
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Indirect method – a two-step process where an unlabeled primary antibody binds to the antigen, followed by a labeled secondary antibody binding to the primary antibody.
The Immunohistochemistry Laboratory at Yazd Reproductive Sciences Institute was established in 2006 to support both basic research and clinical applications, including:
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Locating biomarkers in various tissue types
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Supporting the diagnosis and treatment of cancers
Activities and Equipment
The laboratory is fully equipped with advanced immunohistochemistry and pathology tools, including:
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ABC kit (Goat IgG, Mouse IgG)
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Peroxidase substrate kits (DAB and AEC)
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Avidin/Biotin blocking kit
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Specialized receptor antibodies (types 2, 3, 4, 6, 9)
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Pathology equipment such as tissue processor and microtome
In addition, the laboratory conducts:
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Staining of sperm, eggs, and embryos
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Various specialized tissue staining procedures